Lionel Scaloni named a 27-man squad on Sunday, featuring seven uncapped players including Alexis Mac Allister, Leonardo Balerdi, Nicolas Figal, Lucas Martinez, Nicolas Dominguez, Lucas Ocampos and Adolfo Gaich.
Mac Allister, 20, is on loan at Boca from Premier League club Brighton and Hove Albion, who signed the attacker in January.
Argentina, who finished third at the Copa America, are without superstar Messi as the forward serves a ban.
CONMEBOL opted to suspend Messi for Argentina’s next three matches while hitting him with a $50,000 (£41,000) for his controversial comments.
As a result, Messi will miss the friendly matches against Chile and Mexico as well as an October friendly against Germany and a one-match World Cup qualifying ban.
— Selección Argentina (@Argentina) August 18, 2019
It is an inexperienced squad for the friendlies in September, with Sergio Aguero and Angel Di Maria among the players joining Messi in missing out on the pair of matches.
Argentina are set to take on Chile at the Los Angeles Coliseum on September 5 before taking on Mexico at the Alamodome in San Antonio, Texas five days later.
The fixtures will be Argentina’s first matches since they defeated Chile 2-1 in the Copa America third-place playoff on July 6.
Goalkeepers: Esteban Andrada (Boca Juniors), Franco Armani (River Plate) and Agustin Marchesin (Porto).
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Defenders: Nicolas Otamendi (Manchester City), German Pezzella (Fiorentina), Leonardo Balerdi (Borussia Dortmund), Marcos Rojo (Manchester United), Lucas Martinez Quarta (River Plate), Gonzalo Montiel (River), Nicolas Figal (Independiente), Nicolas Tagliafico (Ajax).
Midfielders: Marcos Acuna (Sporting CP), Leandro Paredes (Paris Saint-Germain), Guido Rodriguez (Club America), Giovani Lo Celso (Tottenham), Nicolas Dominguez (Velez), Rodrigo De Paul (Udinese), Matias Zaracho (Racing), Roberto Pereyra (Watford), Exequiel Palacios (River Plate), Lucas Ocampos (Sevilla), Manuel Lanzini (West Ham), Alexis Mac Allister (Boca).